Ultra-Sensitive Mass Spectrometric Detection of a rEPO Specific O-glycopeptide as an Unambiguous Proof of Doping Follow-up (GOpep2)

Project Title: Ultra-Sensitive Mass Spectrometric Detection of a rEPO Specific O-lycopeptide as an Unambiguous Proof of Doping—Follow-up (GOpep2)

Researchers: Dr. J. Pascual, Dr. J. Segura

Institution: Fundacio IMIM (FIMIM), Spain

Year accepted: 2015

Objectives:

• To improve sample clean-up by using the already developed polyclonal antibody against the peptide backbone and other desalting techniques.
• To improve the nanoLC-MS/MS set-up using monolithic columns for high throughput and on-line sample clean-up.
• To develop monoclonal antibody for future use of the methodology if the use of the polyclonal proves successful.
• To validate the procedure in urine and serum sample