Differentiation between the adminsitarion of the aromatase inhibitor Androstatrienedione, and the anabolic androgenic steroids Boldione and Boldenone

Code: 09A19WS

As per list of the World Anti-Doping Agency (WADA) 2009 Boldenone and Boldione are explicitly listed in group S1 “anabolic androgenic steroids”, and are therefore prohibited in sports, while Androsta-1,4,6-triene-3,17-dione is classified as aromatase inhibitor (class S4. Hormone Antagonists and Modulators, particularised class S4.1. Aromatase Inhibitors). All these three substances are reported to be excreted as Boldenone and/or Boldenone metabolite in the urine. As class S1 substances are considered as “Non-Specified Substances” while class S4.1. substances are judged as “Specified Substances” the assignment to the administered substances is particularly important for the valuation of the adverse analytical finding. Thus, the project aims to investigate the urinary metabolism and pharmacokinetics using mass spectrometric analyses. Isotope ratio mass spectrometry (GC-C-IRMS) will be applied to identify origin of Testosterone and other endogenously occuring steroids. Based on the analytical data criteria to trace the administered substance have to be established. 

Main Findings: 

As per list of the World anti-soping agency (WADA) 2009 Boldenone and Boldione are explicitly listed in group S1 "anabolic androgenic steroids", and are therefore prohibited in sports, while Androsta-1,4,6-treine-3,17-dione is classified as aromatase inhibitor (class S4. Hormone antagonits and modulators, particularised class S4.1 aromatase inhibitors). All these three substance are reported to be excreted as Boldenone and/or Boldenone metabolite in the urine. As class S1 substances are considered as  " non-specified substances" while class S4.1. substances are judged as " specified substances" the assignment to the administered substances is particulary important for the valuation of the adverse analytical finding. Thus, the project aims to investigate the urinary metabolism and pharmacokinetics using mass spectrometric analyses. Isotope ratio mass spectrometry (GTC-C-IRMS) will be applied to identify origin of Testosterone and other endogenously occuring steroids. Based on the analytical data criteria to trace the administered substance have to be established.