Dielectrophoretic Screening for Autologous Blood Transfusions in Endurance Athletes

Code: ISF17A15ZG

our objectives are:

- to reproduce an ABT experiment similar to what athletes are susceptible to perform (small volume of blood: 200ml) and to compare in the same assay reinfusion of refrigerated blood (stored less than one month) or reinfusion of cryopreserved blood. A group of subjects that will donate blood but will not be reinfused will also be integrated as controls.

- to focus on the characterization of small cells (RBC) and microparticles before and after ABT to identify morphological and cell surface markers changes, as well as changes in protein content by proteomic analyses of microparticles.

- to analyse the structural and antigenic properties of band 3, the major protein of the red cell membrane.

We will :

(i) determine if these techniques can efficiently identify an ABT of small volume. And if similar modifications are identified when blood was conserved refrigerated or frozen.

(ii) determine the window of detection of an ABT of small volume with these tests..